Western blot was performed using Anti-CXCL2 Polyclonal Antibody (Product # PA5-80529) and a 7.9 kDa band corresponding to C-X-C motif chemokine 2 was observed LPS treated and secretion blocked HUVEC cells. Whole cell extracts (40 µg lysate) of HUVEC (Lane 1), PTI treated HUVEC (Lane 2), LPS treated HUVEC (Lane 3), LPS and PTI treated HUVEC (Lane 4) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a PVDF membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Uncharacterised bands (*) at ~36 kDa and ~90 kDa was also detected across the panel.